KYTO is a chemically defined, synthetic hydrogel that bears biologically active sites. An MMP-cleavable crosslinker allows for potential cell spreading and migration. The hydrogel can be customised to bear a cell adhesive peptide, RGD, to further fine-tune the properties for specific applications. KYTO increases in viscosity after 8 to 10 minutes, enabling a dense and well-defined cell pattern to be formed followed by enhanced stability of the pattern when the dish is moved from cymatiX to the incubator for complete gelation.
An ideal candidate for custom specific fine-tuning of gel properties
- Tissue Engineering and in vitro models
- In vitro vasculogenesis
- Multicellular systems
- Morphogenesis and tissue formation
The main components of this hydrogel are SG-Dextran and CD-link crosslinker. When combined, these two components form stable thioether bonds. The CD-link bears MMP cleavable sites which allows for potential cell spreading and migration.
At a crosslinking strength of 2 mmol/L and at room temperature, the hydrogel can be used for patterning for 2 to 6 minutes. Complete gelation is achieved within 10 to 30 minutes or by placing the gel in the incubator at 37°C. Crosslinking strength and final mechanical properties can be tuned by varying the SG-Dextran and CD-link concentration.
Gelation kinetics can be adjusted by changing the pH (optional product 10x CB buffer at pH 5.5.). The hydrogel can also be prepared with a thiol-functionalised RGD peptide that covalently binds to the SG-Dextran.
1 box contains 1mL